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Metagenomic approach for studying picoeukaryotes in a extreme oligotrophic marginal sea (South Adriatic Sea) during winter mixed conditions

This dataset that contains primary occurrence data for species.

Description

This study investigates this smallest plankton fraction (cells ≤ 3µm) in both prokaryotic and eukaryotic perspective considering changes of the community in the photic zone from coastal to open sea during mixed winter conditions. Using high-throughput sequencing of 16S and 18S rRNA genes along with flow-cytometry counts of bacteria, cyanobacteria and photosynthetic picoeukaryotes (PPEs) we described picoplanktonic community in the oligotrophic ecosystem. With pigment and lipid analyses of picoplankton community, we characterized PPEs and detected specific events in microbial loop. Prokaryotic community was dominated by Alphaproteobacteria, mainly SAR11 clade (44.91%), followed by Gammaproteobacteria (Oceanospirillales and Pseudomonadales, 14.96%), Bacteroidetes, mainly Flavobacteriales (13%), Cyanobacteria (Prochlorococcus and Synechococcus, 9.52%), Marinimicrobia, SAR406 clade (7.97%), Deltaproteobacteria (3.83%), Actinobacteria (2.24%) and Chloroflexi (1.90%). Picoeukaryotes, although the drivers of photosynthetic activity in oligotrophic systems, in this study were dominated by heterotrophic counterparts: Syndiniales, parasitic dinoflagellates (79.67%), other Dinophyta (8.7%) and on two depths Collodaria, family Sphaerozoidae (22.1%) and Polycistinea, Spumellarida (5.0%), while total photoautotrophic fraction of picoeukaryotes were represented with Mamiellophyceae, Stramenopiles, photoautotrophic Cryptophyta and some Haptophyta, together not exceeding 5% of total sequences. Heterotrophic bacteria and cyanobacteria, separated into populations of Prochlorococcus and Synechococcus, had abundances up to 7×105; 2.3×104 and 2.5×104 cells mL-1, respectively. Photoautotrophic picoeukaryotes were most abundant at P600-25m with 3×103 cells mL-1. According to pigment composition, the most prevalent phototrophs in picoplankton were Cyanobacteria and green algae.

Type of content

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Citation

MGnify (2019). Metagenomic approach for studying picoeukaryotes in a extreme oligotrophic marginal sea (South Adriatic Sea) during winter mixed conditions. Sampling event dataset https://doi.org/10.15468/o39yg1 accessed via GBIF.org on 2024-11-06.

Digitised records

Looking up... the number of records that can be accessed through the BioAtlas - Atlas bioraznolikosti Hrvatske. This resource was last checked for updated data on 14 Nov 2024. The most recent data was published on 14 Nov 2024.

Metadata last updated on 2024-11-14 22:59:00.0

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